Publications


2023

(38) Paracatalytic induction: Subverting specificity in hedgehog protein autoprocessing with small molecules. Ciulla DA, Xu Z, Pezzullo JL, Dranchak P, Wang C, Giner JL, Inglese J, Callahan BP.

Methods Enzymol. 2023;685:1-41.

DOI: 10.1016/bs.mie.2023.03.001.

(37) There's more to enzyme antagonism than inhibition. Callahan BP, Xu Z. Bioorg Med Chem. 2023 Mar 15;82:117231.

DOI: 10.1016/j.bmc.2023.117231.

2022

(36) A cell-based bioluminescence reporter assay of human Sonic Hedgehog protein autoprocessing to identify inhibitors and activators. Ciulla DA, Dranchak P, Pezzullo JL, Mancusi RA, Psaras AM, Rai G, Giner JL, Inglese J, Callahan BP. J Biol Chem. 2022 Dec;298(12):102705.

DOI: 10.1016/j.jbc.2022.102705.

(35) Editorial: Genetically mobile elements repurposed by nature and biotechnologists. Lennon CW, Callahan BP, Cousineau B, Edgell DR, Belfort M. Front Mol Biosci. 2022 Aug 29;9:992664.

DOI: 10.3389/fmolb.2022.992664

(34) Enzymatic Beacons for Specific Sensing of Dilute Nucleic Acid. Zhang X, Kotikam V, Rozners E, Callahan BP. Chembiochem. 2021 Dec 10:e202100594. doi: 10.1002/cbic.202100594.

DOI: 10.1002/cbic.202100594

(33) Nanomolar, Noncovalent Antagonism of Hedgehog Cholesterolysis: Exception to the "Irreversibility Rule" for Protein Autoprocessing Inhibition. Wagner AG, Stagnitta RT, Xu Z, Pezzullo JL, Kandel N, Giner JL, Covey DF, Wang C, Callahan BP. Biochemistry. 2021 Dec 23. DOI: 10.1021/acs.biochem.1c00697

2020

US Patent: US 10,738,338 B2. Callahan BP, Owens TS. METHOD AND COMPOSITION FOR BIOCATALYTIC PROTEIN - OLIGONUCLEOTIDE CONJUGATION AND PROTEIN - OLIGONUCLEOTIDE CONJUGATE. Issued: Aug 11, 2020.

(32) “Specificity Distorted: Chemical Induction of Biological Paracatalysis.” Callahan BP, Ciulla DA, Wagner AG, Xu Z, Zhang X. Biochemistry. 2020 Sep 15. DOI: 10.1021/acs.biochem.0c00643

(31) “Subverting Hedgehog Protein Autoprocessing by Chemical Induction of Paracatalysis.” *Smith CJ, *Wagner AG, Stagnitta RT, Xu Z, Pezzullo JL, Giner JL, Xie J, Covey DF, Wang C, Callahan BP. Biochemistry. 2020 Feb 18;59(6):736-741.*Co-first author DOI: 10.1021/acs.biochem.0c00013

2019

(30) “General Base Swap Preserves Activity and Expands Substrate Tolerance in Hedgehog Autoprocessing.” *Zhao J, *Ciulla DA, Xie J, Wagner AG, Castillo DA, Zwarycz AS, Lin Z, Beadle S, Giner JL, Li Z, Li H, Banavali N, Callahan BP, Wang C. J Am Chem Soc. 2019 Nov 20;141(46):18380-18384. *Co-first author

(29) “Protein-Nucleic Acid Conjugation with Sterol Linkers Using Hedgehog Autoprocessing. Bioconjug Chem.” Zhang X, Xu Z, Moumin DS, Ciulla DA, Owen TS, Mancusi RA, Giner JL, Wang C, Callahan BP. 2019 Nov 20;30(11):2799-2804. (selected for cover) DOI: 10.1021/acs.bioconjchem.9b00550

(28) “Sterol A-ring plasticity in hedgehog protein cholesterolysis supports a primitive substrate selectivity mechanism.” Chem Commun (Camb)Ciulla DA, Wagner AG, Liu X, Cooper CL, Jorgensen MT, Wang C, Goyal P, Banavali NK, Pezzullo JL, Giner JL, Callahan BP. . 2019 55, 1829-1832.

2018

(27) “Chemical Bypass of General Base Catalysis in Hedgehog Protein Cholesterolysis Using a Hyper-Nucleophilic Substrate..”Ciulla, DA, Jorgenson MT, Giner JL, Callahan BP* J. Am. Chem. Soc. (cover image) 2018 140, 916-918. (selected for cover) DOI: 10.1021/jacs.7b05161

2017

(26) “Illuminating Inhibition of Cytochrome P450 17A1: Targeting Prostate Cancer.” Li A., Yadav R., White, J., Herroon, M., Callahan, BP, Podgorski, I., Turro, C., Scott, Emily E., Kodanko, J. ChemComm. 2017 53, 3673-3676.

2016

(25) “Hedgehog proteins consume steroidal CYP17A1 antagonists: Potential therapeutic significance in advanced prostate cancer.” Bordeau B.M., Ciulla D.A., Callahan BP * ChemMedChem. 2016 11, 1983–1986. (Selected for virtual issue, MedChem in USA) DOI: 10.1002/cmdc.201600238

(24) “A single aspartate controls two steps in hedgehog protein autoprocessing.”  Xie J., Owen T., Xia K., Callahan B, Wang C. J. Am. Chem. Soc. 2016 138, 10806-9.

2015

(23) “Hedgehog cholesterolysis: specialized gatekeeper to oncogenic signaling.” Callahan BP *, Wang C.  Cancers (Basel). 2015 7, 2037-53. DOI: https://www.mdpi.com/2072-6694/7/4/875

(22) “Förster resonance energy transfer-based cholesterolysis assay identifies a novel hedgehog inhibitor.” Owen T.S., Ngoje G., Lageman T., Bordeau B., Belfort M, Callahan BP*.  Anal. Biochem. 2015 488, 1-5. DOI: 10.1016/j.ab.2015.06.021

(21) “Zinc inhibits hedgehog autoprocessing: implications for a novel pathway in diseases.” Xie J, Owen TS, Xia K, Vikram-Singh A, Tou E, Li L, Wan LQ, Callahan BP, Wang C. J. Biol. Chem 2015 290: 11591-600. DOI: 10.1074/jbc.M114.623264

(20) “Active site targeting of hedgehog precursor protein with phenylarsine oxide” Owen TS, Xie J, Laraway B, Ngoje G, Wang C, Callahan BP*ChemBioChem. 2015 16:55-8. DOI: 10.1002/cbic.201402421

2014

(19) “Branching out of the intein active site in protein splicing” Callahan BP*, Belfort M*.  Proc. Nat’l Acad. Sci. 2014 111, 8323-4. DOI: 10.1073/pnas.1407116111

(18) (1)H, (13)C, and (15)N NMR assignments of a Drosophila Hedgehog autoprocessing domain. Xie J, Du Z, Callahan B., Belfort M, Wang C. Biomol. NMR Assign. 2014 8, 279-81. DOI: 10.1007/s12104-013-9500-8

Pre-2013

POST-Doctoral Trainee

(17) Dearden AK, Callahan B., Roey PV, Li Z, Kumar U, Belfort M, Nayak SK. A conserved threonine spring-loads precursor for intein splicing.  Protein Sci. 2013 22, 557-63.

(16) Callahan BP*, Stanger M, Belfort M. A redox trap to augment the intein toolbox. Biotechnol Bioeng. 2013 110, 1565-73.

(15) Callahan BP, Topilina, N., Stanger M., Van Roey, P., Belfort M. Structure of a catalytically competent intein caught in a redox trap with functional and biological implications. Nat. Struct. Mol. Biol. 2011 18, 630-3.

(14) Zhang L, Zheng, Y, Callahan B, Belfort M., Liu, Y. Cisplatin as a protein splicing inhibitor suggesting inteins as therapeutic targets in mycobacteria J. Biol. Chem. 2011 286, 1277-82 (Highlighted in Nature Chemical Biology).

(13) Callahan BP, Stanger M., Belfort M. Protease activation of split green fluorescent protein ChemBioChem  2010 11, 2259-63.

(12) Callahan B, Nguyen K., Gormley A., Valdes K., Caplow M., Crossman D.K., Steyn A.J.C., Eisele L., Derbyshire K. Conservation of structure and protein-protein interactions mediated by the secreted mycobacterial proteins EsxA, EsxB, and EspA.  J. Bacteriology. 2010 192, 326-35.

(11) Amitai G., Callahan BP, Stanger M., Belfort G., Belfort M. Modulation of intein activity by its neighboring extein substrates.. Proc. Natl. Acad. Sci. 2009 106, 11005-10.

(10) Coros A., Callahan B, Battaglioli E., Derbyshire K.M. The specialized secretory apparatus ESX-1 is essential for DNA transfer in Mycobacterium smegmatisMol. Microbiol. 2008 69, 794-808.

Graduate Student

(9) Callahan BP *, Miller BG*. OMP decarboxylase—An enigma persists. Bioorg. Chem. 2007 35, 465-9.

(8) Callahan BP, Lomino, J.V., Wolfenden R. Nanomolar inhibition of the enterobactin biosynthesis enzyme, EntE: Synthesis, substituent effects, and additivity. Bioorg. Med. Chem. Lett. 2006 16, 3802-05.

(7) Callahan BP,  Bell,  A.F.,  Tonge  P.,  Wolfenden  R.  A Raman-active competitive inhibitor of OMP decarboxylase Bioorg. Chem. 2006 34, 59-65.

(6) Callahan B.P., Yaun, Y., Wolfenden, R. The Burden Borne by Urease J. Am. Chem. Soc. 2005 127, 10828-9. (Highlighted in Science)

(5) Callahan BP,  Wolfenden,  R.  OMP  Decarboxylase:  an  Experimental  Test  of  Ground  State Destabilization of the Enzyme-Substrate Complex  J. Am. Chem. Soc  2004 126, 14698-9. (Highlighted in Faculty of 1000)

(4) Callahan BP, Wolfenden, R. Charge Development in the Transition State for Decarboxylations in Water: Spontaneous and Acetone-Catalyzed Decarboxylation of Aminomalonate J. Am. Chem. Soc. 2004 126, 4514-15. (Highlighted in Faculty of 1000)

(3) Callahan BP, Wolfenden, R. Migration of Methyl Groups between Aliphatic Amines in Water J. Am. Chem. Soc. 2003 125, 310-11.

Laboratory Technician

(2)  Wei C., Callahan BP, Turner M.J., Willis R.A., Lord E.M., Barth R.K., Frelinger J.G. Regulation of human prostate-specific antigen gene expression in transgenic mice: evidence for an enhancer between the PSA and human glandular kallikrein-1 genes. Int J Mol Med. 1998 2, 487-96.

(1) Willis R.A., Wei C., Turner M.J., Callahan BP, Pugh A.E., Barth R.K., Lord E.M., Frelinger J.G. A transgenic strategy for analyzing the regulatory regions of the human prostate-specific antigen gene: potential applications for the treatment of prostate cancer (Review). Int J Mol Med. 1998 1, 379-86.